讲座题目：Sensing and mapping DNA methylation 主讲人：Matthias Bochtler 教授 主持人🦚：翁杰敏 教授 开始时间：2019-10-14 10:00:00 讲座地址#️⃣：生命科学学院534小会议室 主办单位：生命科学学院
报告人简介🥺： 1990–1992：Studies in physics at the LMU Munich 1992–1993🧑🏽🦰：Guest student at the University of Cambridge, UK 1993–1995🥕：Studies in physics at the LMU Munich. Diploma with distinction 1996–1999：PhD at the Max Planck Institute of Biochemistry in Martinsried (Germany) under Prof. Robert Huber (“summa cum laude”) 1999–2000♟🙎🏼♂️：Postdoc at the Max Planck Institute of Biochemistry in Martinsried 2001-present：Head of a joint MPG-PAN Junior Research Group at the International Institute of Molecular and Cell Biology (IIMCB) in Warsaw 2005/2006 Habilitation in biochemistry at the Institute of Bioorganic Chemistry (IChB) PAN in Poznan (submission 2005, official confirmation 2006). 2007-2011🤳🏻：Head of Structural Biology, Cardiff University (United Kingdom) 2009：Professorial title awarded by the President of the Republic of Poland 2011-present Group Leader at IIMCB and professor at the Institute of Biochemistry and Biophysics (IBB) of the Polish Academy of Sciences 报告内容🧑🎨🛐： Enzymatic DNA methylation comes in three main varieties, as as 5-methylcytosine, 4-methylcytosine, and 6-methyladenine. In the first part of my talk, I will report crystallographic and bioinformatic data on how DNA methylation is sensed by proteins, both negatively and positively. I will show that there is a small modular toolkit for this task, and that cytosine and adenine methylation are detected rather differently. In the second part of my talk, I will describe our efforts to characterize maintenance of DNA methylation in a model organism, the plant A. thaliana, by bisulfite conversion and reversible terminator (Illumina) sequencing, and by Nanopore sequencing. The data indicate strong long range correlations in methylation of a single DNA molecule, and provide evidence for crosstalk between different methylation types (CpG, CHG, CHH) |
